The association of the Thr715Pro P-selectin genotype with levels of P-selectin in platelet concentrates.

Abstract

BACKGROUND AND OBJECTIVES P-selectin is stored in the alpha granules of platelets and in the Weibel Palade bodies of endothelial cells; upon activation, it is translocated to the cell surface and released into the plasma in soluble form. One variant of the P-selectin gene, the Thr715Pro polymorphism, is strongly associated with the plasma levels of soluble P-selectin. In platelet concentrates soluble P-selectin can be regarded mainly platelet derived. MATERIALS AND METHODS The relation of the genotype with soluble P-selectin, platelet expressed P-selectin and the sum of all forms of P-selectin - comprising soluble P-selectin, platelet surface P-selectin and P-selectin from the alpha granules - was assessed in fresh whole blood and in apheresis platelets suspended in 35% plasma/65% SSP+ obtained from 89 platelet donors. RESULTS Levels of total P-selectin were genotype associated (P = 0·025); likewise, in fresh whole blood there was an association of soluble P-selectin with genotype (P = 0·02). In platelets suspended in additive solution, however, levels of the storage-associated or TRAP-6 agonist induced increase of platelets' P-selectin were not associated with the genotype. A correlation between levels of soluble P-selectin and surface expression of P-selectin was observed on day 3 of storage in Thr715Thr individuals (P < 0·0001), but not in heterozygotes (Thr715Pro, P = 0·2). CONCLUSION The donors' genotype has only little influence on levels of soluble P-selectin in apheresis platelets suspended in 35% plasma/65% SSP+.

DOI: 10.1111/vox.12175

Cite this paper

@article{JilmaStohlawetz2014TheAO, title={The association of the Thr715Pro P-selectin genotype with levels of P-selectin in platelet concentrates.}, author={Petra Jilma-Stohlawetz and C H Mannhalter and Alexandra Kaider and Theresa Waidacher and Bernd Jilma and Simon Panzer}, journal={Vox sanguinis}, year={2014}, volume={107 4}, pages={368-74} }