The assessment of cartilage degradation in vivo: development of an immunoassay for the measurement in body fluids of type II collagen cleaved by collagenases.

@article{Poole2004TheAO,
  title={The assessment of cartilage degradation in vivo: development of an immunoassay for the measurement in body fluids of type II collagen cleaved by collagenases.},
  author={A. Robin Poole and Mirela Ionescu and M A Fitzcharles and R. Clark Billinghurst},
  journal={Journal of immunological methods},
  year={2004},
  volume={294 1-2},
  pages={145-53}
}
A monoclonal antibody has been developed which recognizes a neoepitope in type II collagen which is generated by the intrahelical cleavage of collagenases. Antibody reactivity is directed at the carboxyl-terminus of the TCA or 3/4 piece of the degraded alpha1(II) chain. Reactivity is dependent upon hydroxylation of proline. Evidence is provided suggesting… CONTINUE READING