The art and design of functional metagenomic screens.


This article summarizes general design principles for functional metagenomics. The focus is on Escherichia coli as an expression host, although alternative host-vector systems are discussed in relation to optimizing gene recovery in activity-based screens. Examples of DNA isolation and enrichment approaches, library construction and phenotypic read-out are… (More)
DOI: 10.1016/j.copbio.2011.02.010


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@article{Taupp2011TheAA, title={The art and design of functional metagenomic screens.}, author={Marcus Taupp and Keith Mewis and Steven J. Hallam}, journal={Current opinion in biotechnology}, year={2011}, volume={22 3}, pages={465-72} }