The argininosuccinate lyase gene of Chlamydomonas reinhardtii : cloning of the cDNA and its characterization as a selectable shuttle marker

@article{Auchincloss1999TheAL,
  title={The argininosuccinate lyase gene of Chlamydomonas reinhardtii : cloning of the cDNA and its characterization as a selectable shuttle marker},
  author={Andrea H. Auchincloss and A. I. Loroch and J.-D. Rochaix},
  journal={Molecular and General Genetics MGG},
  year={1999},
  volume={261},
  pages={21-30}
}
We describe the cDNA sequence for ARG7, the gene that encodes argininosuccinate lyase – a selectable nuclear marker – in Chlamydomonas reinhardtii. The 5′ end of the cDNA contains one more exon and the organisation of the mRNA is different from that predicted from the genomic sequence. When expressed under the control of the endogenous RbcS2 promoter, the 2.22-kb cDNA complements the arg7 mutation as well as the genomic DNA. A linear cDNA fragment lacking promoter sequences is also able to… CONTINUE READING