The application of peptide nucleic acid probes for rapid detection and enumeration of eubacteria, Staphylococcus aureus and Pseudomonas aeruginosa in recreational beaches of S. Florida.

@article{Esiobu2004TheAO,
  title={The application of peptide nucleic acid probes for rapid detection and enumeration of eubacteria, Staphylococcus aureus and Pseudomonas aeruginosa in recreational beaches of S. Florida.},
  author={Nwadiuto Esiobu and Renuka Mohammed and Andrea Echeverry and Melissa Green and Tonya D Bonilla and Aaron Hartz and D. J. McCORQUODALE and Andrew Rogerson},
  journal={Journal of microbiological methods},
  year={2004},
  volume={57 2},
  pages={157-62}
}
A novel chemiluminescent in situ hybridization technique using peptide nucleic acids (PNA) was adapted for the detection of bacteria in beach sand and recreational waters in South Florida. The simultaneous detection and enumeration of eubacteria and the novel indicators, Staphylococcus aureus and Pseudomonas aeruginosa, was achieved within 6-8 h of processing. Following 5 h of incubation on TSA, soybean peroxidase-labeled peptide nucleic acid probes (Boston Probes, Boston, MA) targeting species… CONTINUE READING

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