The application of flow cytometry for estimating genome size and ploidy level in plants.

@article{Pellicer2014TheAO,
  title={The application of flow cytometry for estimating genome size and ploidy level in plants.},
  author={Jaume Pellicer and Ilia J. Leitch},
  journal={Methods in molecular biology},
  year={2014},
  volume={1115},
  pages={279-307}
}
Over the years, the amount of DNA in a nucleus (genome size) has been estimated using a variety of methods, but increasingly, flow cytometry (FCM) has become the method of choice. The popularity of this technique lies in the ease of sample preparation and in the large number of particles (i.e., nuclei) that can be analyzed in a very short period of time. This chapter presents a step-by-step guide to estimating the nuclear DNA content of plant nuclei using FCM. Attempting to serve as a tool for… CONTINUE READING
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