The application of an in situ karyotyping technique for mesenchymal stromal cells: a validation and comparison study with classical G-banding

@inproceedings{Hwang2013TheAO,
  title={The application of an in situ karyotyping technique for mesenchymal stromal cells: a validation and comparison study with classical G-banding},
  author={Sang Mee Hwang and Cha-ja See and Jungeun Choi and Seon Young Kim and Qute Choi and Jung Ah Kim and Jiseok Kwon and Si Nae Park and Kyongok Im and Il-Hoan Oh and Dong Soon Lee},
  booktitle={Experimental & Molecular Medicine},
  year={2013}
}
The cytogenetic analysis of mesenchymal stromal cells (MSCs) is essential for verifying the safety and stability of MSCs. An in situ technique, which uses cells grown on coverslips for karyotyping and minimizes cell manipulation, is the standard protocol for the chromosome analysis of amniotic fluids. Therefore, we applied the in situ karyotyping technique in MSCs and compared the quality of metaphases and karyotyping results with classical G-banding and chromosomal abnormalities with… CONTINUE READING