The application of a novel thrombin sensitive site in genetic engineering.

@article{Hong1997TheAO,
  title={The application of a novel thrombin sensitive site in genetic engineering.},
  author={M Hong and Wu-yan Chen and Shengdong Lu},
  journal={Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih},
  year={1997},
  volume={12 3},
  pages={143-7}
}
To obtain high efficiency of cleavage of thrombin in fusion protein containing alpha-ANP fused to Ref peptides, the linker sequence designed as VIAGR which was different from GVRGPR formerly used was studied. Plasmid pHL carrying the fusion gene Ref-NT-ANP downstream from PL promoter was derived from expression vector pLY1 by inserting the fragment of NT-ANP into it. The expression of fusion gene was induced at 42 degrees C, and the interested protein Ref-NT-ANP accumulated as inclusion bodies… CONTINUE READING

From This Paper

Topics from this paper.
0 Citations
0 References
Similar Papers

Similar Papers

Loading similar papers…