The application of CRISPR-Cas for single species identification from environmental DNA.

@article{Williams2019TheAO,
  title={The application of CRISPR-Cas for single species identification from environmental DNA.},
  author={Molly A. Williams and Joyce O'Grady and Bernard Ball and Jens Carlsson and Elvira de Eyto and Philip McGinnity and Eleanor Jennings and Fiona Regan and Anne Parle‐McDermott},
  journal={Molecular ecology resources},
  year={2019}
}
We report the first application of CRISPR-Cas technology to single species detection from environmental DNA (eDNA). Organisms shed and excrete DNA into their environment such as in skin cells and faeces, referred to as environmental DNA (eDNA). Utilising eDNA allows non-invasive monitoring with increased specificity and sensitivity. Current methods primarily employ PCR-based techniques to detect a given species from eDNA samples, posing a logistical challenge for on-site monitoring and… 

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