The application of 3D micropatterning of agarose substrate for cell culture and in situ comet assays.


We report the fabrication of a 3D micropatterned agarose substrate that enables the culture of single or multiple cells. Patterning was performed on dried agarose using deep UV irradiation leading to 6-microm-deep micropatterns of 25-70 microm in diameter. Cell adhesion was facilitated by the specific grafting of ECM (extra cellular matrix) proteins such as… (More)
DOI: 10.1016/j.biomaterials.2010.01.020


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