The appearance of arginine and arginase in pumpkin cotyledons. Characterization of arginase

@inproceedings{Splittstoesser1969TheAO,
  title={The appearance of arginine and arginase in pumpkin cotyledons. Characterization of arginase},
  author={Walter E. Splittstoesser},
  year={1969}
}
Abstract During germination an increase in free arginine and arginase activity occurs in the cotyledons of pumpkin seedlings grown either in the light or dark, with the increase in enzyme activity paralleling the increase in free arginine content. Arginase was purified 9-fold from etiolated cotyledons and has a pH optimum of 9·5 in pyrophosphate buffer. The velocity of the reaction was linear up to 2 hr with a requirement for manganese. Iron, cobalt and copper gave a slight stimulation while… CONTINUE READING