The apparent involvement of vibrio fetus in an infection of man.

  • Brant Ward
  • Published 1948 in Journal of bacteriology

Abstract

Many articles can be found in the literature concerning the infection of livestock by Vibrio fetus. References have been made to such infections in both the male and female animal. To the knowledge of this writer, however, only one report has been made concerning infections with this organism, or at least a very similar one, in the human race. Curtis (1913) reported the isolation of a curved, motile, anaerobic bacillus from uterine discharges of two female patients: one whose infection followed instrumental abortion, and the other whose labor at full term was complicated by the same organism. This rod Curtis assigned to the genus Vibrio. Apparently no reports have been made of a vibrionic infection in the male human involving this or any very similar organism. Recently a male worker in this laboratory, in an attempt to obtain a pure culture of a Staphylococcus for addition to the stock culture collection, had occasion to open aseptically a small, simple pustule located on the cheek. From the pus and exudate obtained, nutrient agar and blood-enriched (10 per cent) nutrient agar plates were inoculated by the streak method. At the same time a film was made from this material, which was stained by Gram's method. This preparation showed "comma" or "S-shaped" gram-negative organisms, a few short gram-negative rods, and large, nonsporulating, gram-positive rods. Culturally the large rod grew quite as well on plain nutrient agar as on the blood medium under aerobic conditions and at a temperature of 37 C. Growth was visible in 24 hours. The small, curved, gram-negative rods, however, were reluctant to grow even upon the 10 per cent blood agar. Transfer to a fresh plate of blood agar under an atmosphere of 10 per cent CO2, however, produced an increased amount of growth in 3 to 4 days. Colonies of the large rod were 5 to 15 mm in diameter, raised, concave, glistening, smooth, entire, and dirtywhite in color. Colonies of the small, curved rod were 1 to 3 mm in diameter, raised, concave, glistening, smooth, entire, and blue to blue-gray in color. Growth of this second organism was also obtained upon inoculation of thioglycolate blood broth.2 This growth was noted to occur at a depth of one-half inch. To one set of thioglycolate blood broth tubes, 3 per cent gelatin was 1 The investigation reported in this paper is in connection with a project of the Wyoming Agricultural Experiment Station and is published by permission of the Director. 2 This consisted of: Neopeptone............... 10 g Gelatin.............. 1 g K2HPO4.... 2 g Meat extract ......................... 3 g Sodium thioglycolate............. 1 g Water...... 1,000 ml Agar 1 g Adjusted to pH 7.0

Cite this paper

@article{Ward1948TheAI, title={The apparent involvement of vibrio fetus in an infection of man.}, author={Brant Ward}, journal={Journal of bacteriology}, year={1948}, volume={55 1}, pages={113-4} }