The apolipoprotein B gene is constitutively expressed in HepG2 cells: regulation of secretion by oleic acid, albumin, and insulin, and measurement of the mRNA half-life.

@article{Pullinger1989TheAB,
  title={The apolipoprotein B gene is constitutively expressed in HepG2 cells: regulation of secretion by oleic acid, albumin, and insulin, and measurement of the mRNA half-life.},
  author={Clive R. Pullinger and Julia D. North and B B Teng and Vincent A Rifici and A E Ronhild de Brito and Julie Denise Scott},
  journal={Journal of lipid research},
  year={1989},
  volume={30 7},
  pages={1065-77}
}
The objective of this study was to establish conditions whereby apoB secreted from HepG2 cells could be regulated over a wide range, and to determine whether changes of output were correlated with the level of apoB mRNA. The presence of oleate (complexed to 3% albumin at a molar ratio of 1.7:1) resulted in a 3.5-fold stimulation of apoB secretion that was apparent after only 3 h. Insulin halved the rate of apoB output and the inhibition was detectable within the physiological insulin range, but… CONTINUE READING
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