The antisuppressor strain sin1 of Schizosaccharomyces pombe lacks the modification isopentenyladenosine in transfer RNA.

@article{Janner1980TheAS,
  title={The antisuppressor strain sin1 of Schizosaccharomyces pombe lacks the modification isopentenyladenosine in transfer RNA.},
  author={F Janner and Guillem Viladot Voegeli and Rudolf Fluri},
  journal={Journal of molecular biology},
  year={1980},
  volume={139 2},
  pages={
          207-19
        }
}
The modified base N6-(Δ2-isopentenyl)-adenosine (i6A) is missing in all transfer RNAs isolated from the antisuppressor strain sin1 of Schizosaccharomyces pombe. i6A is found adjacent to the 3′ side of the anticodon of several tRNAs of S. pombe. Sequence analysis of tyrosine tRNA from the antisuppressor strain sin1 shows an unmodified adenosine instead of the i6A. i6A-deficient tyrosine tRNA elutes much earlier than wild-type tRNATyr during reverse phase chromatography (RPC-5). Serine tRNA and… CONTINUE READING