The antiherpes drug (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdU) interferes with formation of N-linked and of O-linked oligosaccharides of the herpes simplex virus type 1 glycoprotein C.

@article{Olofsson1985TheAD,
  title={The antiherpes drug (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdU) interferes with formation of N-linked and of O-linked oligosaccharides of the herpes simplex virus type 1 glycoprotein C.},
  author={S. Olofsson and M. Lundstr{\"o}m and R. Datema},
  journal={Virology},
  year={1985},
  volume={147 1},
  pages={
          201-5
        }
}
In HSV-1 infected cell the antiherpes drug (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVdU) exerted at least three different effects on glycosylation of glycoprotein gC. First, an overall decrease of protein glycosylation occurred due to inhibition of synthesis of the lipid-linked oligosaccharides, precursors of N-linked oligosaccharides of gC. Second, an inhibition of processing of N-linked oligosaccharides occurred after the acquisition of endo H-resistance, and possibly due to inhibition of… Expand
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References

SHOWING 1-10 OF 15 REFERENCES
Effect of (E)-5-(2-bromovinyl)-2'-deoxyuridine on synthesis of herpes simplex virus type 1-specific polypeptides
TLDR
The alterations in HSV-1 polypeptide expression appear to be due to the presence of the drug in a low-molecular-weight form as well as its presence in the viral DNA, which suggests that this analog or a phosphorylated derivative might act as an inhibitor of an enzyme(s) responsible for posttranslational modification ofpolypeptides. Expand
Inhibition of glycosylation of bovine herpesvirus 1 glycoproteins by the thymidine analog (E)-5-(2 Bromovinyl)-2'-deoxyuridine.
TLDR
The results suggest that BVdU partially inhibits the glycosylation of BHV-1 glycoproteins, and immature neutral and basic precursors of the mature viral glyCoproteins accumulated. Expand
The relationship between incorporation of E-5-(2-Bromovinyl)-2'-deoxyuridine into herpes simplex virus type 1 DNA with virus infectivity and DNA integrity.
TLDR
The potent antiviral action of BrvdUrd observed in cell culture correlates not only with its incorporation into HSV-1 DNA but also with an altered stability of this DNA. Expand
Endo-beta-N-acetylglucosaminidase H sensitivity of precursors to herpes simplex virus type 1 glycoproteins gB and gC
TLDR
The endoglycosidase endo-beta-N-acetylglucominidase H (endo H) was used to examine the nature of the oligosaccharides associated with the herpes simplex virus type 1 glycoproteins gA, gB, and gC, and it was suggested that gB contained both complex- and simple-type oligosACcharides, andgA contained only simple-types. Expand
Effect of energy depletion on the glycosylation of a viral glycoprotein.
TLDR
Both inhibition of formation of mannosyl-phosphoryl-dolichol and of intracellular migration are reserved when the energy status is brought back to normal levels, and the rate of oligosaccharide-processing are decreased in the energy-depleted cells. Expand
Glycoprotein C of herpes simplex virus type 1: characterization of O-linked oligosaccharides.
In contrast to other viral glycoproteins, the herpes simplex virus (HSV) glycoprotein C(gC) binds to the N-acetylgalactosamine-specific Helix pomatia lectin (HPA). In the present paper gC wasExpand
The lipid pathway of protein glycosylation and its inhibitors: the biological significance of protein-bound carbohydrates.
TLDR
Although 2-deoxy- d -arabinose-hexose has been shown mainly to affect glycosylation (in the systems studied so far), its mode of action in more complex, biological systems may not always depend on its well known property. Expand
The structural basis of the different affinities of two types of acidic N‐glycosidic glycopeptides for concanavalin a—sepharose
TLDR
It was found that glycopeptides possessing two peripheral NeuNAcGal-GlcNAc*-branches linked to the core pentasaccharide were bound by the lectin, whereas glycopePTides with three branches were not (the structures of these compounds are shown below). Expand
Nucleoside-diphosphate derivatives of 2-deoxy-D-glucose in animal cells.
After administration of 14C-labelled 2-deoxy-d-glucose or 3H-labelled nucleosides to cells in tissue culture the following activated intermediates of the deoxysugar have been isolated andExpand
Fractionation of asparagine-linked oligosaccharides by serial lectin-Agarose affinity chromatography. A rapid, sensitive, and specific technique.
TLDR
It is concluded that serial lectin-agarose affinity chromatography is a rapid, sensitive, and specific technique for fractionating and analyzing Asn-linked oligosaccharides. Expand
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