The antigen-binding site of an N-propionylated polysialic acid-specific antibody protective against group B meningococci is consistent with extended epitopes.

@article{Johal2013TheAS,
  title={The antigen-binding site of an N-propionylated polysialic acid-specific antibody protective against group B meningococci is consistent with extended epitopes.},
  author={Asha R. Johal and Harold C. Jarrell and James A. Letts and Nam Huan Khieu and Roxanne C Landry and Wojciech Jachymek and Qingling Yang and Harold J. Jennings and Jean Robert Brisson and Stephen V Evans},
  journal={Glycobiology},
  year={2013},
  volume={23 8},
  pages={
          946-54
        },
  url={https://api.semanticscholar.org/CorpusID:9755772}
}
The model of extended NPrPSA decasaccharide bound to 13D9 derived from docking studies is consistent with saturation transfer difference nuclear magnetic resonance experiments, providing further evidence that extended epitopes have the ability to break immune tolerance associated with the polysialic acid capsule of these pathogens.
View on PubMed
academic.oup.com
8 Citations
Background Citations
1

Figures and Tables from this paper

8 Citations

NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies.

Synthesis and immunological characterization of modified hyaluronic acid hexasaccharide conjugates.

Immunological studies in mice show very similar antibody responses with both conjugates, suggesting that the N-acetyl groups of the glucosaminyl residues of the HA-hexasaccharide are not a critical part of the epitope recognized by the anti-HA polyclonal immune response.

Antibody recognition of carbohydrate epitopes†.

This review summarizes structural features of anti-carbohydrate antibodies to over 20 antigens, based on six categories of glyco-antigen, including the glycan shield of HIV glycoproteins and a group of antibodies to Kdo-containing Chlamydia epitopes.

Antibody-Mediated Catalysis in Infection and Immunity

It is suggested that antibody-mediated catalysis is a fertile area for study with broad applications in infection and immunity, where processes often occur over much longer times and involve high antibody concentrations

Using crystallographic water properties for the analysis and prediction of lectin-carbohydrate complex structures.

The results show that solvent structure adjacent to the binding sites mimics the ligand oxygen structural framework in the resulting protein-ligand complex, allowing us to develop a predictive method using a Naive Bayes classifier and show how these properties can be used to improve docking predictions of lectin-carbohydrate complex structures in terms of both accuracy and precision.

Biophysical Characterization of Polysialic Acid—Membrane Nanosystems

The analysis, based on the Goldman-Hodgkin-Katz equation, of trans Membrane potential changes resulting from trans-membrane translocation of polySia is described and its ability to modulate membrane surface charge density, pH at the membrane surface, and membrane potentials is described.

Chemical synthesis of α(2,8) octasialosides, the minimum structure of polysialic acids.

Chemical synthesis of an α(2,8) octasialic acid by using an N-acetyl-5-N,4-O:7-O,9-O-dicarbonyl protected sialyl donor is reported. The glycosyl donor underwent α-selective sialylation at the C8…

NMR‐Assisted Molecular Docking Methodologies

The state of the art of docking methods which have been recently bridged to NMR experiments to identify novel and effective therapeutic drug candidates are summarized.

57 References

The epitope associated with the binding of the capsular polysaccharide of the group B meningococcus and of Escherichia coli K1 to a human monoclonal macroglobulin, IgMNOV

The fine structure of the combining site of human mAb IgMNOV to poly- alpha(2----8)linked NeuNAc, the epitope of the group B meningococcal and E. coli K1 polysaccharides, has been probed using RIA and ELISA and indicates that both IgMNov and H46 have groove-type sites that bind exclusively to an epitope located on the acidic side of the inner residues.

Crystallization and preliminary X-ray diffraction analysis of antigen-binding fragments which are specific for antigenic conformations of sialic acid homopolymers.

The antigen-binding fragments of one protective and one non-protective monoclonal antibody specific for the capsular polysaccharides of group B meningococci have been crystallized and have undergone preliminary X-ray diffraction analysis.

Determinant specificities of the groups B and C polysaccharides of Neisseria meningitidis.

The nature of the colominic acid-specific group B determinant was elucidated by a radioimmunoassay inhibition technique with the use of a series of linear alpha-(2----8)-linked oligomers of sialic acid as inhibitors.

Unique intermolecular bactericidal epitope involving the homosialopolysaccharide capsule on the cell surface of group B Neisseria meningitidis and Escherichia coli K1.

The above experiments not only effected a separation of highly bactericidal antibodies but also provided evidence that the long spacer arm is functional in the binding of the bactericidal antibody to the affinity column, which supports the hypothesis that the epitope is polysaccharide-associated and is probably intermolecular in nature.

N-Propionylated Group B Meningococcal Polysaccharide Mimics a Unique Bactericidal Capsular Epitope in Group B Neisseria meningitidis

The bactericidal epitope mimicked by the NPrGBMP was shown to be ubiquitous in the capsule of both GBM and E. coli K1 using immunogold labeling techniques and, because of its unique properties, its identification could be significant in the development of a comprehensive conjugate vaccine against group B meningococcal meningitis.

Exploring the mimicry of polysaccharide antigens by anti-idiotypic antibodies. The crystallization, molecular replacement, and refinement to 2.8 A resolution of an idiotope-anti-idiotope Fab complex and of the unliganded anti-idiotope Fab.

The monoclonal antibody YsT 9.1 is specific for the lipopolysaccharide A antigen of Brucella abortus and a complex formed between the Fab of YsT9.1 (Ab1) and theFab of its antidiotopic monoconal antibody T91AJ5 (Ab2) has been crystallized, an example of "induced fit" of antigen to antibody.

Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance.

Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2-->8)-polysialic acid.

X-ray crystallographic and thermodynamic evidence are consistent with a binding site that accommodates at least eight sialic acid residues, and a model of antigen binding is presented that satisfies this thermodynamic data, as well as a previously reported requirement of conformational specificity of the oligosaccharide.

Helical epitope of the group B meningococcal alpha(2-8)-linked sialic acid polysaccharide.

The immunological properties of the group B meningococcal alpha(2-8)-linked sialic acid polysaccharide have been rationalized in terms of a model where the random coil nature of the polymer can be…

Immunochemistry of groups A, B, and C meningococcal polysaccharide-tetanus toxoid conjugates.

Hyperimmunization of mice with the groups A and C polysaccharide-tetanus toxoid conjugate produced antisera with good bactericidal activity against their respective homologous organisms, and indicated the potential of these conjugates as potential human vaccines.
...