The anti‐inflammatory actions of methotrexate are critically dependent upon the production of reactive oxygen species

@article{Phillips2003TheAA,
  title={The anti‐inflammatory actions of methotrexate are critically dependent upon the production of reactive oxygen species},
  author={Darren Charles Phillips and Kevin J. Woollard and Helen Griffiths},
  journal={British Journal of Pharmacology},
  year={2003},
  volume={138}
}
The mechanism of action by which methotrexate (MTX) exerts its anti‐inflammatory and immunosuppressive effects remains unclear. The aim of this study is to investigate the hypothesis that MTX exerts these effects via the production of reactive oxygen species (ROS). Addition of MTX (100 nM–10 μM) to U937 monocytes induced a time and dose dependent increase in cytosolic peroxide [peroxide]cyt from 6–16 h. MTX also caused corresponding monocyte growth arrest, which was inhibited (P<0.05) by pre… Expand
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  • 2018
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