The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes.

@article{Haruki2008TheAT,
  title={The anchor-away technique: rapid, conditional establishment of yeast mutant phenotypes.},
  author={Hirohito Haruki and Junichi Nishikawa and U. K. Laemmli},
  journal={Molecular cell},
  year={2008},
  volume={31 6},
  pages={925-32}
}
The anchor-away (AA) technique depletes the nucleus of Saccharomyces cerevisiae of a protein of interest (the target) by conditional tethering to an abundant cytoplasmic protein (the anchor) by appropriate gene tagging and rapamycin-dependent heterodimerization. Taking advantage of the massive flow of ribosomal proteins through the nucleus during maturation, a protein of the large subunit was chosen as the anchor. Addition of rapamycin, due to formation of the ternary complex, composed of the… CONTINUE READING
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