The -amylase of the Beetle Callosobruchus chinensis. Properties.

  title={The -amylase of the Beetle Callosobruchus chinensis. Properties.},
  author={H Podoler and Shalom W. Applebaum},
  journal={The Biochemical journal},
  volume={121 2},
C. chinensis larval amylase is activated by Ca(2+) and inhibited by Cl(-) and EDTA (K(i) 6.7x10(-3)m). GSH and 2-mercaptoethanol activate, presumably at different sites, as 2-mercaptoethanol interferes with Ca(2+) activation, whereas GSH enhances it. The inhibition by iodoacetic acid and N-ethylmaleimide (K(i) 1.55x10(-2)m) suggest that free thiol groups are essential for activity. The pH optimum of 5.2-5.4 is moved to 5.6-5.8 by Ca(2+) and 2-mercaptoethanol. The activation energy is 7270 cal… CONTINUE READING

From This Paper

Topics from this paper.


Publications citing this paper.
Showing 1-9 of 9 extracted citations

Isolation and partial characterization of three isoamylases of Rhyzopertha dominica F. (Coleoptera: Bostrichidae).

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology • 2008
View 3 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…