The amoeba-to-flagellate transformation test is not reliable for the diagnosis of the genus Naegleria. Description of three new Naegleria spp.

@article{deJonckheere2001TheAT,
  title={The amoeba-to-flagellate transformation test is not reliable for the diagnosis of the genus Naegleria. Description of three new Naegleria spp.},
  author={Johan F. de Jonckheere and S. Brown and Philip J. Dobson and B S Robinson and Pierre Pernin},
  journal={Protist},
  year={2001},
  volume={152 2},
  pages={
          115-21
        }
}
Trophozoites of several isolates from one location in Australia have failed consistently to transform into flagellates, although they display all other characteristics of the genus Naegleria. When changing the standard transformation test, flagellates were produced. In phylogenetic trees derived from partial small subunit ribosomal DNA (SSUrDNA) sequences, one of these strains branches close to a cluster comprising N. clarki, N. australiensis, N. italica and N. jadini. It is proposed that these… 
A Century of Research on the Amoeboflagellate Genus Naegleria
TLDR
The state of affairs concerning species designation based on phylogeny in the genus Acanthamoeba, another free-living amoeba with species pathogenic to man, is discussed and two lineages are discussed and identified as separate species.
Morphology and Phylogenetic Analyses of Three Novel Naegleria Isolated from Freshwaters on Jeju Island, Korea, During the Winter Period
TLDR
Three novel Naegleria were isolated from freshwaters on Jeju Island, Korea, during winter, and the phylogenetic analyses consistently revealed that the isolates are members of the NaEGleria group, however, the isolate differ from other species in both phylogenetic trees.
Identification of free-living amoebae and amoeba-associated bacteria from reservoirs and water treatment plants by molecular techniques.
TLDR
The results highlight the importance of amoebae in natural waters as reservoirs of potential pathogens and its possible role in the spread of bacterial genera with interest in public and environmental health.
Description of a New Species with a Remarkable Cyst Structure in the Genus Naegleria: Naegleria angularis sp. n.
TLDR
Ribosomal DNA sequence analysis showed that the isolate belongs to the genus Naegleria, that it represents a novel species and that its closest relative is N. pussardi.
A New Halophilic Heterolobosean Flagellate, Aurem hypersalina gen. n. et sp. n., Closely Related to the Pleurostomum‐Tulamoeba Clade: Implications for Adaptive Radiation of Halophilic Eukaryotes
TLDR
This isolate of a biflagellate from a hypersaline water of 342‰ salinity shared several morphological features with typical halophilic heterolobosean flagellates, indicating that this new clade is composed almost entirely of obligate Halophilic taxa.
A New Heterolobosean Amoeboflagellate, Tetramitus dokdoensis n. sp., Isolated from a Freshwater Pond on Dokdo Island in the East Sea, Korea
TLDR
The strain isolated from the Dokdo Island is proposed as a novel species, Tetramitus dokdoensis n.
...
...

References

SHOWING 1-10 OF 21 REFERENCES
Low Genetic Diversity In Willaertia Magna From Wide Geographical Sources, and Characterisation of Willaertia Minor N. Sp. (Heterolobosea, Vahlkampfidae)
TLDR
Cysts and trophozoites are more readily mistaken for Naegleria, but lack perinuclear granules, as do the mature cysts of W. magna, and an additional isolate assigned to Willaertia by features of its flagellate and cyst stages represents a new species.
Biochemical identification and phylogenetic relationships in free-living amoebas of the genus Naegleria.
TLDR
The genetic data suggest that there are two main phylogenetic groups in the genus Naegleria, and certain enzymes proved particularly useful from a practical point of view as they allow easy and reliable identification of pathogenic N. fowleri and N. lovaniensis strains.
Three different group I introns in the nuclear large subunit ribosomal DNA of the amoeboflagellate Naegleria.
We have amplified the large subunit ribosomal DNA (LSUrDNA) of the 12 described Naegleria spp. and of 34 other Naegleria lineages that might be distinct species. Two strains yielded a product that is
Flagella number among Naegleria flagellates.
TLDR
Although the majority of flagellates had 2 flagella, there was considerable variation among individual cells, and Naegleria jadini and N. gruberi were intermediate with 80% and 74% biflageLLates, respectively.
...
...