The amino acid sequence and phosphorylation sites of a boar transition protein 1.

@article{Akama1994TheAA,
  title={The amino acid sequence and phosphorylation sites of a boar transition protein 1.},
  author={Kuniko Akama and Somei Kojima and Masahiro Nakano and Toranosuke Tobita and Hideyuki Hayashi},
  journal={Biochemistry and molecular biology international},
  year={1994},
  volume={32 2},
  pages={349-57}
}
Boar transition protein 1 was extracted with acid from the testes, purified by chromatographies on CM-Sephadex C-25 and Sephadex G-50, and reduced and carboxymethylated. The modified protein was purified by HPLC on Nucleosil 300 7C18. The primary structure of the protein was determined by automated Edman degradation of the C-terminal peptide of the BrCN-cleaved protein and of the whole protein, and by carboxypeptidase digestion of it. The study of phosphorylation sites showed that Ser36 and… CONTINUE READING

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