The alpha1b-adrenoceptor exists as a higher-order oligomer: effective oligomerization is required for receptor maturation, surface delivery, and function.

@article{LpezGimnez2007TheAE,
  title={The alpha1b-adrenoceptor exists as a higher-order oligomer: effective oligomerization is required for receptor maturation, surface delivery, and function.},
  author={Juan Francisco L{\'o}pez-Gim{\'e}nez and Meritxell Canals and John D. Pediani and Graeme Milligan},
  journal={Molecular pharmacology},
  year={2007},
  volume={71 4},
  pages={1015-29}
}
Approaches to identify G protein-coupled receptor oligomers rather than dimers have been lacking. Using concatamers of fluorescent proteins, we established conditions to monitor sequential three-color fluorescence resonance energy transfer (3-FRET) and used these to detect oligomeric complexes of the alpha(1b)-adrenoceptor in single living cells. Mutation of putative key hydrophobic residues in transmembrane domains I and IV resulted in substantial reduction of sequential 3-FRET and was… CONTINUE READING
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