The alpha-amylase gene amyH of the moderate halophile Halomonas meridiana: cloning and molecular characterization.

@article{Coronado2000TheAG,
  title={The alpha-amylase gene amyH of the moderate halophile Halomonas meridiana: cloning and molecular characterization.},
  author={M J Coronado and Carmen Vargas and Encarnaci{\'o}n Mellado and Georgios Tegos and Constantin Drainas and Joaqu{\'i}n J. Nieto and Antonio Ventosa},
  journal={Microbiology},
  year={2000},
  volume={146 ( Pt 4)},
  pages={861-8}
}
Two types of Tn1732-induced mutants defective in extracellular amylase activity were isolated from the moderate halophile Halomonas meridiana DSM 5425. Type I mutants displayed amylase activity in the periplasm, and were unable to use any of the carbon sources tested, including starch and its hydrolysis product maltose. The type II mutant was affected in the gene responsible for the synthesis of the extracellular alpha-amylase. This gene (amyH) was isolated by functional complementation of… CONTINUE READING

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