The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis

@article{Buttner1987TheAG,
  title={The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis},
  author={Mark J. Buttner and Ian M. Fearnley and Mervyn Bibb},
  journal={Molecular and General Genetics MGG},
  year={1987},
  volume={209},
  pages={101-109}
}
The DNA sequence of a 1.77 kb region of the Streptomyces coelicolor chromosome containing the coding and regulatory regions of the extracellular agarase (dagA) gene was determined. The sequence predicts a primary translation product of 309 amino acids and Mr 35132. Comparison of the N-terminal sequence determined for the mature extracellular protein with that of the primary translation product deduced from the DNA sequence predicts the presence of a 30 amino acid signal peptide. Analysis of the… CONTINUE READING

8 Figures & Tables

Connections & Topics

Mentioned Connections BETA
Analysis of the transcription of the dagA gene using high resolution S1 mapping , in vitro transcription , dinucleotide - primed in vitro transcription and in vivo promoter probing identified four promoters , initiating transcription approximately 32 , 77 , 125 and 220 nucleotides upstream of the coding sequence .
All Topics