The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal.

Abstract

Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection Lowering pHi further by incubation with acetate increases the sensitivity of cells to MG.

Showing 1-10 of 10 extracted citations

Statistics

0200400'98'00'02'04'06'08'10'12'14'16
Citations per Year

394 Citations

Semantic Scholar estimates that this publication has received between 43 and 1,450 citations based on the available data.

See our FAQ for additional information.