The activities of acidic and glutamine-rich transcriptional activation domains in plant cells: design of modular transcription factors for high-level expression

@article{Schwechheimer1998TheAO,
  title={The activities of acidic and glutamine-rich transcriptional activation domains in plant cells: design of modular transcription factors for high-level expression},
  author={Claus Schwechheimer and Caroline Smith and Michael W. Bevan},
  journal={Plant Molecular Biology},
  year={1998},
  volume={36},
  pages={195-204}
}
The aim of this work was to design strong transcriptional activators that can be used to regulate plant gene expression. The contribution of different components in a transcription factor and target gene system was assayed by measuring transcriptional activation. Each component was optimised to achieve maximal reporter gene expression in transient protoplast transformation assays. The DNA-binding domain of the yeast transcriptional activator GAL4 was studied in the context of fusion proteins… CONTINUE READING
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