The active site of purple acid phosphatase from sweet potatoes (Ipomoea batatas) metal content and spectroscopic characterization.

@article{Durmus1999TheAS,
  title={The active site of purple acid phosphatase from sweet potatoes (Ipomoea batatas) metal content and spectroscopic characterization.},
  author={Ali Durmus and Christoph Eicken and B H Sift and A Kratel and Reinhard Kappl and J{\"u}rgen H{\"u}ttermann and Bernt Krebs},
  journal={European journal of biochemistry},
  year={1999},
  volume={260 3},
  pages={709-16}
}
Purple acid phosphatase from sweet potatoes Ipomoea batatas (spPAP) has been purified to homogeneity and characterized using spectroscopic investigations. Matrix-assisted laser desorption/ionization mass spectrometry analysis revealed a molecular mass of approximately 112 kDa. The metal content was determined by X-ray fluorescence using synchrotron radiation. In contrast to previous studies it is shown that spPAP contains a Fe(III)-Zn(II) center in the active site as previously determined for… CONTINUE READING