The activation of P2Y6 receptor in cultured spinal microglia induces the production of CCL2 through the MAP kinases-NF-κB pathway.

@article{Morioka2013TheAO,
  title={The activation of P2Y6 receptor in cultured spinal microglia induces the production of CCL2 through the MAP kinases-NF-κB pathway.},
  author={Norimitsu Morioka and Masato Tokuhara and Sakura Harano and Yoki Nakamura and Kazue Hisaoka-Nakashima and Yoshihiro Nakata},
  journal={Neuropharmacology},
  year={2013},
  volume={75},
  pages={116-25}
}
Rat primary cultures of spinal microglia were stimulated by UTP, a known P2Y2/4 receptor agonist, which resulted in the production and release of the C-C chemokine CCL2 (monocyte chemoattractant protein-1; MCP-1) measured by real-time PCR and ELISA, respectively. In an in vitro preparation of rat spinal microglia, with regard to the P2Y subtypes, the expression of P2Y1, 2, 6, 12, 13 and P2Y14, but not P2Y4, were detected by RT-PCR. The subtype of microglial P2Y receptor which could be involved… CONTINUE READING