The action of proteolytic enzymes on N,N-dimethyl proteins. Basis for a microassay for proteolytic enzymes.

@article{Lin1969TheAO,
  title={The action of proteolytic enzymes on N,N-dimethyl proteins. Basis for a microassay for proteolytic enzymes.},
  author={Yi-Do. Lin and Gary E. Means and Robert Earl Feeney},
  journal={The Journal of biological chemistry},
  year={1969},
  volume={244 3},
  pages={
          789-93
        }
}
Abstract N,N-Dimethyl proteins were prepared by reductive methylation of their amino groups with formaldehyde and NaBH4. Proteolysis of these alkylated proteins by trypsin, α-chymotrypsin, subtilisin, pepsin, and fungal protease was determined by direct measurement of the bonds split, with the use of trinitrobenzenesulfonic acid to determine directly the appearance of new terminal amino groups. The low blank values obtained with N,N-dimethyl proteins has resulted in a greatly increased… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 17 CITATIONS

Inactivation of alveolar macrophage transglutaminase by oxidants in cigarette smoke.

  • Journal of leukocyte biology
  • 1986
VIEW 2 EXCERPTS
CITES METHODS
HIGHLY INFLUENCED

Similar Papers

Loading similar papers…