The acrosome reaction-inducing activity of individual human follicular fluid samples is highly variable and is related to the steroid content.

@article{Morales1992TheAR,
  title={The acrosome reaction-inducing activity of individual human follicular fluid samples is highly variable and is related to the steroid content.},
  author={Patricio Garrido Morales and Miguel Llanos and Gabriela Adela G{\'a}nem Guti{\'e}rrez and P. R. Kohen and P. Alonso Vigil and David Vantman},
  journal={Human reproduction},
  year={1992},
  volume={7 5},
  pages={
          646-51
        }
}
In this study, we have evaluated the relationship between the acrosome reaction-inducing activity of individual human follicular fluid samples and their steroid content. Eighteen samples of follicular fluid were obtained during egg retrieval in six patients undergoing assisted fertilization. Motile spermatozoa were incubated in modified Tyrode's medium (26 mg/ml bovine serum albumin) for 20 h at 1 x 10(7) cells/ml. In a single experiment, aliquots of a semen specimen were simultaneously treated… CONTINUE READING
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