The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation.

@article{Hardy1968TheAA,
  title={The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation.},
  author={Robert William Hardy and R D Holsten and E K Jackson and R. C. Burns},
  journal={Plant physiology},
  year={1968},
  volume={43 8},
  pages={1185-207}
}
The methodology, characteristics and application of the sensitive C(2)H(2)-C(2)H(4) assay for N(2) fixation by nitrogenase preparations and bacterial cultures in the laboratory and by legumes and free-living bacteria in situ is presented in this comprehensive report. This assay is based on the N(2)ase-catalyzed reduction of C(2)H(2) to C(2)H(4), gas chromatographic isolation of C(2)H(2) and C(2)H(4), and quantitative measurement with a H(2)-flame analyzer. As little as 1 mumumole C(2)H(4) can… CONTINUE READING
Highly Influential
This paper has highly influenced 30 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 372 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 13 references

The biochemistry and postulated mechanisms of N 2 fixation

  • R. W. F. HARDY, E. KNIGHT, AND E. K. JACK-SON
  • 1968

Acety - lene as a competitive inhibitor of N 9 fixation

  • W. S SILVER
  • Proc . Natl . Acad . Sci . U . S .
  • 1967

Biological reductions catalyzed by symbiotic nitrogen - fixing tissues

  • W. D. P STEWART
  • 1967

Substrate versatility of nitrogenase

  • AND R. H. BURRIS
  • 1967

Study of intermediates in nitrogen fixation

  • R. AND R. H. BURRIS SCHOLLHORN
  • Federation Proc .
  • 1966

Evaluation of incoming and outgoing processes that affect soil nitrogen

  • F. E. Clark
  • 1965

Nitrogen fixation studies witlh aerobic and photosynthetic bacteria

  • San Pietro, R. C. BURNS
  • Non - Heme Iron Proteins : Role in Energy…
  • 1965

Similar Papers

Loading similar papers…