The accumulation of polymorphonuclear leukocytes in post-ischemic skeletal muscle in the rat, measured by quantitating tissue myeloperoxidase.


In the present study we have evaluated the activity of myeloperoxidase (MPO) in ischemic reperfused skeletal muscle of rats, as an index of polymorphonuclear leukocyte (PMNL) accumulation and its time course. A tourniquet model for temporary ischemia was used, in which one hindleg was made ischemic for 1.5, 3 or 5 hours. Muscle biopsies were taken after 12… (More)