The acceleration of cardiomyogenesis in embryonic stem cells in vitro by serum depletion does not increase the number of developed cardiomyocytes

  title={The acceleration of cardiomyogenesis in embryonic stem cells in vitro by serum depletion does not increase the number of developed cardiomyocytes},
  author={Katarzyna Anna Radaszkiewicz and Dominika S{\'y}korov{\'a} and Lucia Bin{\'o} and Jana Kudov{\'a} and Mark{\'e}ta B{\'e}barov{\'a} and Jiřina Proch{\'a}zkov{\'a} and Hana Kotasov{\'a} and Luk{\'a}{\vs} Kubala and Jiř{\'i} Pachern{\'i}k},
  journal={PLoS ONE},
The differentiation of pluripotent embryonic stem (ES) cells into various lineages in vitro represents an important tool for studying the mechanisms underlying mammalian embryogenesis. It is a key technique in studies evaluating the molecular mechanisms of cardiomyogenesis and heart development and also in embryotoxicology. Herein, modest modifications of the basic protocol for ES cell differentiation into cardiomyocytes were evaluated in order to increase the yield and differentiation status… Expand
Opioid receptors and opioid peptides in the cardiomyogenesis of mouse embryonic stem cells
The overall data do not support the notion that opioid peptides have a significant potential to promote the spontaneous cardiomyogenesis of mES cells in vitro. Expand
Neural crest related gene transcript regulation by valproic acid analogues in the cardiac embryonic stem cell test.
Biomarkers from dedicated mechanistic pathways, e.g. NC differentiation, provide promising tools for monitoring specific effects in ESTc, and STITCH (‘search tool for interactions of chemicals’) analysis showed relationships between the examined signalling pathways and suggested additional candidate marker genes. Expand
12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC/ERK signaling
It is shown that TPA can be applied as a cardiomyogenesis-promoting factor for the differentiation of mouse embryonic stem (mES) cells in vitro and postulate that a TPA-sensitive population of cardiac progenitor cells exists at a certain time point and that the proposed treatment can be used to increase the multiplication of ES cell-derivedCardiomyocytes. Expand
The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells.
It is reported that p38alpha kinase-deficient embryonic stem cells exhibit a higher production of reactive oxygen species (ROS) in contrast to their wild-type counterpart, and analysis of the expressions of NADPH oxidases and dual oxidases shows NOX2/gp91phox is over-expressed in p38 alpha deficient cells. Expand
Conducting composite films based on chitosan or sodium hyaluronate. Properties and cytocompatibility with human induced pluripotent stem cells.
The combination of conductivity, antibacterial activity and mainly cytocompatibility with hiPSC opens wide application potential of these polysaccharide-based composites. Expand
The biocompatibility of polyaniline and polypyrrole: A comparative study of their cytotoxicity, embryotoxicity and impurity profile.
There is a bigger difference between the biocompatibility of the respective polymers in their salt and base forms than between PANI and PPy as such and PANI can, therefore, be similarly applied in biomedicine when solely their biological properties are considered. Expand


Effect of bone morphogenetic protein-4 ( BMP4 ) on cardiomyocyte differentiation from mouse embryonic stem cell
The present study was designed to evaluate the effect of BMP-4 on mouse embryonic stem cells (ESCs)-derived cardiomyocyte. Cardiac differentiation of the mouse ESCs was initiated by embryoid bodiesExpand
Effect of bone morphogenetic protein-4 (BMP-4) on cardiomyocyte differentiation from mouse embryonic stem cell.
BMP-4 had an inhibitory effect on cardiomyocyte differentiation from the mouse ESCs in terms of ultrastructural characteristics, genes expression and functional properties. Expand
Increased Cardiomyocyte Differentiation from Human Embryonic Stem Cells in Serum‐Free Cultures
A striking inverse relationship between cardiomyocyte differentiation and the concentration of FCS is demonstrated during HES‐2‐END‐2 coculture, and this model represents an improved in vitro model, without interfering factors in serum, for testing other factors that might promote cardiomeocyte differentiation. Expand
Wnt3a‐Induced Mesoderm Formation and Cardiomyogenesis in Human Embryonic Stem Cells
A novel differentiation method to substantially increase hESCM yield is reported, which has the potential to guarantee a renewable supply of enriched cardiomyocyte populations for developing novel and more predictive cell models, and should provide valuable insights into pathways critical for cardiac regeneration. Expand
Differentiation of Pluripotent Embryonic Stem Cells Into Cardiomyocytes
This review summarizes the current state of ES cell–derived cardiogenesis and provides an overview of how genomic strategies coupled with this in vitro differentiation system can be applied to cardiac research. Expand
Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts.
A simple genetic manipulation can be used to select essentially pure cultures of cardiomyocytes from differentiating ES cells that are suitable for the formation of intracardiac grafts, and should be applicable to all ES-derived cell lineages. Expand
Generation and characterization of cardiomyocytes under serum-free conditions.
It is concluded that the use of serum replacement media (SRM) in the authors' experiments led to more specific differentiation but reduced proliferation because these SRMs contained reduced essential substances like growth factors and hormones. Expand
DMH1, a Novel BMP Small Molecule Inhibitor, Increases Cardiomyocyte Progenitors and Promotes Cardiac Differentiation in Mouse Embryonic Stem Cells
DMH1 differs significantly from its predecessor by its ability to enrich for pro-cardiac progenitor cells that respond to late-stage Wnt inhibition using XAV939 and produce secondary beating cardiomyocytes, demonstrating the utility of small molecules to complement existing in vitro cardiac differentiation protocols. Expand
HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells
It is demonstrated that HIF-1α deficiency significantly altered the quality and quantity of mESC-derived cardiomyocytes and was accompanied with lower mRNA and protein levels of cardiac cell specific markers and with a decreasing percentage of myosin heavy chain α and β, and cardiac troponin T-positive cells. Expand
STAT3-Dependent Mouse Embryonic Stem Cell Differentiation Into Cardiomyocytes: Analysis of Molecular Signaling and Therapeutic Efficacy of Cardiomyocyte Precommitted mES Transplantation in a Mouse Model of Myocardial Infarction
It is suggested that LIF and BMP-2 may synergistically enhance CMC differentiation of transplanted stem cells and downstream signaling components or cell type specific precommitment may facilitate the effects of ES cell–based therapies for post-MI myocardial repair and regeneration. Expand