The WHO multicenter study on Listeria monocytogenes subtyping: random amplification of polymorphic DNA (RAPD).

@article{Wernars1996TheWM,
  title={The WHO multicenter study on Listeria monocytogenes subtyping: random amplification of polymorphic DNA (RAPD).},
  author={Karel Wernars and Patrick Boerlin and Andr{\'e} Audurier and Eric G. Russell and Gordon D W Curtis and Lieve Herman and Nathalie L. van der Mee-Marquet},
  journal={International journal of food microbiology},
  year={1996},
  volume={32 3},
  pages={325-41}
}
As part of a WHO multicenter study on Listeria monocytogenes subtyping methods the random amplification of polymorphic DNA (RAPD)-technique was evaluated. Six participants were asked to use a standard protocol to analyse a set of 80 L. monocytogenes strains. This set contained 22 groups of epidemiologically linked isolates and 11 pairs of duplicate strains. Using three different 10-mer primers the median reproducibility of the RAPD-results obtained by the six participants was 86.5% (range 0-100… CONTINUE READING

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