The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants.

@article{Bak2003TheVP,
  title={The VirD2 pilot protein of Agrobacterium-transferred DNA interacts with the TATA box-binding protein and a nuclear protein kinase in plants.},
  author={L{\'a}szl{\'o} Bak{\'o} and Masaaki Umeda and Antonio Fern{\'a}ndez Tiburcio and J. L. Schell and Csaba Koncz},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2003},
  volume={100 17},
  pages={10108-13}
}
The bacterial virulence protein VirD2 plays an important role in nuclear import and chromosomal integration of Agrobacterium-transferred DNA in fungal, plant, animal, and human cells. Here we show that in nuclei of alfalfa cells, VirD2 interacts with and is phosphorylated by CAK2Ms, a conserved plant ortholog of cyclin-dependent kinase-activating kinases. CAK2Ms binds to and phosphorylates the C-terminal regulatory domain of RNA polymerase II largest subunit, which can recruit the TATA box… CONTINUE READING