The Vancomycin biosynthetic pathway is induced in oxygen-limited Amycolatopsis orientalis (ATCC 19795) cultures that do not produce antibiotic.

@article{Dunstan2000TheVB,
  title={The Vancomycin biosynthetic pathway is induced in oxygen-limited Amycolatopsis orientalis (ATCC 19795) cultures that do not produce antibiotic.},
  author={Dunstan and Avignone-Rossa and P Langley and Bushell},
  journal={Enzyme and microbial technology},
  year={2000},
  volume={27 7},
  pages={502-510}
}
Vancomycin biosynthetic capacity was stimulated in oxygen-limited bioreactor culture (6.4% DOT) of Amycolatopsis orientalis, coinciding with a down-regulation of the culture growth and protein synthesis rates. However no Vancomycin was detected. ATP production was similar in oxygen-limited and oxygen-sufficient cultures (400-150 µmole(ATP). g(biomass)(-1)). Our findings suggest a critical requirement for oxygen flux through the Vancomycin biosynthetic pathway, which is only met under nutrient… CONTINUE READING