The VP2/VP3 minor capsid protein of simian virus 40 promotes the in vitro assembly of the major capsid protein VP1 into particles.

@article{Kawano2006TheVM,
  title={The VP2/VP3 minor capsid protein of simian virus 40 promotes the in vitro assembly of the major capsid protein VP1 into particles.},
  author={Masa-aki Kawano and Takamasa Inoue and Hiroko Tsukamoto and Tatsuya Takaya and Teruya Enomoto and Ryou-u Takahashi and Naoki Yokoyama and Noriaki Yamamoto and Akira Nakanishi and Takeshi Imai and Tadashi Wada and Kohsuke Kataoka and Hiroshi Handa},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 15},
  pages={10164-73}
}
The SV40 capsid is composed primarily of 72 pentamers of the VP1 major capsid protein. Although the capsid also contains the minor capsid protein VP2 and its amino-terminally truncated form VP3, their roles in capsid assembly remain unknown. An in vitro assembly system was used to investigate the role of VP2 in the assembly of recombinant VP1 pentamers. Under physiological salt and pH conditions, VP1 alone remained dissociated, and at pH 5.0, it assembled into tubular structures. A… CONTINUE READING