The Utility of Whole Genome Amplification for Typing Compromised Forensic Samples

@article{Barber2006TheUO,
  title={The Utility of Whole Genome Amplification for Typing Compromised Forensic Samples},
  author={Amy L. Barber and David R. Foran},
  journal={Journal of Forensic Sciences},
  year={2006},
  volume={51}
}
ABSTRACT: Biological evidence has become invaluable in the crime laboratory; however, it may exist in limited quantity and/or quality. Given this, the ability to amplify total DNA obtained from evidence, in an unbiased manner, would be highly advantageous. Methods for whole genome amplification (WGA) have the potential to fulfill this role, resulting in a virtually unlimited supply of DNA. In the research presented, two WGA methods, improved primer extension preamplification and multiple… 
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49 Citations
Highly Influential Citations
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Background Citations
17
Methods Citations
9
Results Citations
4

49 Citations

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Citation Type

Whole genome amplification of degraded and nondegraded DNA for forensic purposes
TLDR
Seven whole genome amplification methods were chosen, investigated, and compared to evaluate their ability to “recover” degraded and nondegraded DNA, and the best results were obtained with GenomePlex which successfully amplified also severely degraded DNA, thus enabling correct typing of mtDNA and Y-SNP loci.
Whole Genome Amplification
TLDR
Although WGA methods have yet to demonstrate their utility for routine forensic analysis, selective genomic enrichment and the production of sufficient sample for multiple repeat analyses are possible novel applications.
Whole genome amplification and real-time PCR in forensic casework
TLDR
Comparative analysis of amplified and genomic DNA samples showed that a large number of SNPs could be accurately typed starting from just 0.01 ng of template, suggesting that MDA can also generate large amounts of genome-equivalent DNA from a minimal amount of input DNA.
Forensic Applications of Whole Genome Amplification
TLDR
A review of the literature was carried out in order to assess the latest research and to identify a potentially fit for purpose whole genome amplification technique.
Document Title: Development and Evaluation of a Whole Genome Amplification Method for Accurate Multiplex STR Genotyping of Compromised Forensic Casework Samples
TLDR
The dcDOP-PCR method significantly improved STR allele success when compared to traditional STR analysis (without pre-amplification), producing strong partial or full profiles in many cases where little to no STR data was previously obtained.
Title : Assessing Deep Sequencing Technology for Human Forensic Mitochondrial DNA Analysis
TLDR
This effort evaluated two newly emerging methods of DNA sequence analysis to obtain massively parallel mtDNA sequence information (deep sequencing) from hair, buccal, and blood samples and demonstrated that careful design of fusion PCR primers supports the creation of amplified targets ready for deep sequencing on both the Roche GS-JuniorTM and Illumina® platforms.
Multiple strand displacement amplification of mitochondrial DNA from clinical samples
TLDR
Genetic screening of mtDNA amplified by WGA is applicable for the detection of cancer associated mutations and the feasibility of this method for increasing the amount of DNA available for analysis, recovering the identical mtDNA sequence, and accurately detecting mtDNA point mutations associated with cancer are shown.
Evaluating the effects of whole genome amplification strategies for amplifying trace DNA using capillary electrophoresis and massive parallel sequencing.
Sequence analysis for HV I region of mitochondrial DNA using WGA (Whole Genome Amplification) method.
Evaluation of circular DNA substrates for whole genome amplification prior to forensic analysis.
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