The Use of a GroEL-BLI Biosensor to Rapidly Assess Preaggregate Populations for Antibody Solutions Exhibiting Different Stability Profiles.

@article{Pace2018TheUO,
  title={The Use of a GroEL-BLI Biosensor to Rapidly Assess Preaggregate Populations for Antibody Solutions Exhibiting Different Stability Profiles.},
  author={Samantha E Pace and Sangeeta B. Joshi and Reza Esfandiary and Robert Stadelman and Steven M. Bishop and C. Russell Middaugh and Mark T. Fisher and David B. Volkin},
  journal={Journal of pharmaceutical sciences},
  year={2018},
  volume={107 2},
  pages={
          559-570
        }
}
An automated method using biotinylated GroEL-streptavidin biosensors with biolayer interferometry (GroEL-BLI) was evaluated to detect the formation of transiently formed, preaggregate species in various pharmaceutically relevant monoclonal antibody (mAb) samples. The relative aggregation propensity of various IgG1 and IgG4 mAbs was rank ordered using the GroEL-BLI biosensor method, and the least stable IgG4 mAb was subjected to different stresses including elevated temperatures, acidic pH, and… CONTINUE READING

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