The Use of Formalin Fixation in the Cytochemical Demonstration of Succinic and Dpn- and Tpn-dependent Dehydrogenases in Mitochondria

@article{Walker1963TheUO,
  title={The Use of Formalin Fixation in the Cytochemical Demonstration of Succinic and Dpn- and Tpn-dependent Dehydrogenases in Mitochondria},
  author={Donald G. Walker and Arnold M. Seligman},
  journal={The Journal of Cell Biology},
  year={1963},
  volume={16},
  pages={455 - 469}
}
Brief formalin fixation in the cold prior to histochemical assay of rat liver and pancreas for various dehydrogenases has been used successfully to circumvent the structural damage and enzymatic loss to which mitochondria of frozen sections would otherwise be subject. To obtain an optimal result a single set of conditions has been devised, including fixation prior to freezing of minute (finely diced) organ blocks in graded concentrations (0.7 to 2.0 per cent) of formaldehyde in chilled (1-4… CONTINUE READING

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A simple procedure for optimal preservation of mitochondria and oxidative enzymes in frozen sections

  • Monis
  • J. Histochem. and Cytochem
  • 1963

Cytological studies by the Altmann-Gersh freezing-drying method

  • N L Hoerr
  • Anat. Rec
  • 1936

FIGVRE ~3 Cytochemical preparations of the pancreas after chilled formalin-BSS fixation are uniformly of high quality regardless of which ditetrazole is used. Lactate-DPN-nitro-BT medium

  • FIGVRE ~3 Cytochemical preparations of the…
  • 1000

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