The Use of Fatty Acid Esters to Enhance Free Acid Sophorolipid Synthesis

  title={The Use of Fatty Acid Esters to Enhance Free Acid Sophorolipid Synthesis},
  author={Richard D. Ashby and Daniel K. Y. Solaiman and Thomas A. Foglia},
  journal={Biotechnology Letters},
Fatty acid esters were prepared by transesterification of soy oil with methanol (methyl-soyate, Me-Soy), ethanol (ethyl-soyate, Et-Soy) and propanol (propyl-soyate, Pro-Soy) and used with glycerol as fermentation substrates to enhance production of free-acid sophorolipids (SLs). Fed-batch fermentations of Candida bombicola resulted in SL yields of 46 ± 4 g/l, 42 ± 7 g/l and 18 ± 6 g/l from Me-Soy, Et-Soy, and Pro-Soy, respectively. Liquid chromatography with atmospheric pressure ionization mass… CONTINUE READING


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