The Use of Coded PCR Primers Enables High-Throughput Sequencing of Multiple Homolog Amplification Products by 454 Parallel Sequencing

@article{Binladen2007TheUO,
  title={The Use of Coded PCR Primers Enables High-Throughput Sequencing of Multiple Homolog Amplification Products by 454 Parallel Sequencing},
  author={Jonas Binladen and Marcus Thomas Pius Gilbert and Jonathan P. Bollback and Frank Panitz and Christian Bendixen and R. Nielsen and Eske Willerslev},
  journal={PLoS ONE},
  year={2007},
  volume={2},
  pages={376 - 380}
}
BACKGROUND The invention of the Genome Sequence 20 DNA Sequencing System (454 parallel sequencing platform) has enabled the rapid and high-volume production of sequence data. Until now, however, individual emulsion PCR (emPCR) reactions and subsequent sequencing runs have been unable to combine template DNA from multiple individuals, as homologous sequences cannot be subsequently assigned to their original sources. METHODOLOGY We use conventional PCR with 5'-nucleotide tagged primers to… CONTINUE READING
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