The Unfinished Story of Cytochrome f

  title={The Unfinished Story of Cytochrome f},
  author={D. Bendall},
  journal={Photosynthesis Research},
  • D. Bendall
  • Published 2004
  • Chemistry, Medicine
  • Photosynthesis Research
Cytochrome f is a unique, integral membrane protein. The background to its discovery by Robert Hill (1899–1991) and Ronald Scarisbrick over 60 years ago and the influence of David Keilin (1887–1963) and Frederick Gowland Hopkins (1861–1947) are discussed. The development of methods for isolating cytochrome f is outlined, emphasizing the remarkable achievement of Hill and Scarisbrick at a time when few if any membrane proteins had been isolated, and the importance of the discovery of a natural… Expand
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Derek Bendall (1930–2014)
Derek Bendall carried out pioneering work on photosynthetic electron transport, particularly on protein–protein interactions, cytochromes, and cyclic electron transport, as well as on other topicsExpand


The preparation and some properties of cytochrome f
  • H. Davenport, R. Hill
  • Chemistry, Medicine
  • Proceedings of the Royal Society of London. Series B - Biological Sciences
  • 1952
Evidence is presented that in addition to iron-protein bonds there are stable linkages between protein and the tetrapyrrol ring resembling those of cytochrome c, and the possible significance of cy tochrome f in the oxidation-reductions of the chloroplast reaction is discussed. Expand
Purification and properties of monomeric cytochrome f from charlock, Sinapis arvensis L.
  • J. Gray
  • Chemistry, Medicine
  • European journal of biochemistry
  • 1978
Monomeric cytochrome f has been purified to homogeneity, as judged by polyacrylamide gel electrophoresis, isoelectric focusing and analytical ultracentrifugation, from the leaves of charlock, SinapisExpand
Role of charges on cytochrome f from the cyanobacterium Phormidium laminosum in its interaction with plastocyanin.
The kinetic evidence from this study appears to conflict with the NMR structure of the P. laminosum complex, which suggests the absence of electrostatic interactions in the final complex, and the most likely explanation of the apparent paradox is that the overall rate is diffusion controlled and that electrostatics specifically influence the encounter complex and not the reaction complex. Expand
The History of Cell Respiration and Cytochrome
  • S. Goldhor
  • Medicine
  • The Yale Journal of Biology and Medicine
  • 1966
The history of cell respiration from Galen and Harvey, Lavoisier and Pasteur, to the now classic but then misunderstood work of MacMunn, and from Keilin's own early work through to the comparatively recent discoveries of the properties and structure of cytochromes is an intensely personal history. Expand
Plastocyanin cytochrome f interaction.
Peptide mapping and sequencing studies revealed two sites of linkage between the two proteins, and Asp-44 of plastocyanin is covalently linked to Lys-187 of cytochrome f, which represents the first identification of a group on cy tochrome f that is involved in the interaction with plastOCyanin. Expand
Some characteristics of cytochrome f in the cyanobacterium Phormidium laminosum: its sequence and charge properties in the reaction with plastocyanin.
Comparisons suggested that the local charges on P. laminosum cytochrome f that are important for its interaction with the homologous plastocyanin may be negative rather than positive, and the importance of acidic groups was confirmed. Expand
Partial Resolution of the Enzymes Catalyzing Photophosphorylation
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The role of acidic residues of plastocyanin in its interaction with cytochrome ƒ.
Results provide convincing evidence for participation of residues of both acidic patches of spinach plastocyanin in the interaction with cytochrome ƒ. Expand
Reactivity of cytochromes c and f with mutant forms of spinach plastocyanin.
The reduction of plastocyanin by cytochromes c and f has been investigated with mutants of spinach plastOCyanin in which individual, highly conserved surface residues have been modified, and a surprising result was that the kinetics of reduction of Asp42Asn were not significantly different from wild type, despite the loss of a negative charge. Expand
[33] Cytochrome components in chloroplasts of the higher plants
Because cytochrome f is confined to chloroplasts, it would appear advantageous that the starting material should be isolated chloroplast or their fragments freed by washing from cytoplasmic proteins, soluble chloroplast proteins, vacuolar acids, and phenolics. Expand