The U11/U12 snRNP 65K protein acts as a molecular bridge, binding the U12 snRNA and U11‐59K protein

  title={The U11/U12 snRNP 65K protein acts as a molecular bridge, binding the U12 snRNA and U11‐59K protein},
  author={H. Benecke and R. L{\"u}hrmann and C. Will},
  journal={The EMBO Journal},
  • H. Benecke, R. Lührmann, C. Will
  • Published 2005
  • Biology, Medicine
  • The EMBO Journal
  • U11 and U12 interact cooperatively with the 5′ splice site and branch site of pre‐mRNA as a stable preformed di‐snRNP complex, thereby bridging the 5′ and 3′ ends of the intron within the U12‐dependent prespliceosome. To identify proteins contributing to di‐snRNP formation and intron bridging, we investigated protein–protein and protein–RNA interactions between components of the U11/U12 snRNP. We demonstrate that the U11/U12‐65K protein possesses dual binding activity, interacting directly with… CONTINUE READING
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