The Structure of the Eukaryotic Ribosome at 3.0 Å Resolution

  title={The Structure of the Eukaryotic Ribosome at 3.0 {\AA} Resolution},
  author={Adam Ben-Shem and Nicolas Garreau de Loubresse and Sergey V. Melnikov and Lasse B. Jenner and Gulnara Yusupova and Marat Yusupov},
  pages={1524 - 1529}
A close-up view of the ribosome’s 79 proteins and 5500 RNA nucleotides. Ribosomes translate genetic information encoded by messenger RNA into proteins. Many aspects of translation and its regulation are specific to eukaryotes, whose ribosomes are much larger and intricate than their bacterial counterparts. We report the crystal structure of the 80S ribosome from the yeast Saccharomyces cerevisiae—including nearly all ribosomal RNA bases and protein side chains as well as an additional protein… 
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This review highlights the past decade's structural work on eukaryotic ribosomes and its implications on the understanding of eUKaryotic translation.
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Structure of the 30S ribosomal subunit
The crystal structure of the 30S subunit from Thermus thermophilus, refined to 3 Å resolution, is reported, which will facilitate the interpretation in molecular terms of lower resolution structural data on several functional states of the ribosome from electron microscopy and crystallography.
Crystal Structure of the Eukaryotic Ribosome
The crystal structure of the yeast 80S ribosome determined at 4.15 angstrom resolution reveals the higher complexity of eukaryotic ribosomes, which are 40% larger than their bacterial counterparts.
Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution
Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES–ES and r-protein–ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
Crystal Structure of the Ribosome at 5.5 Å Resolution
The crystal structure of the complete Thermus thermophilus 70S ribosome containing bound messenger RNA and transfer RNAs (tRNAs) at 5.5 angstrom resolution is described, suggesting coupling of the 20 to 50 angstrom movements associated with tRNA translocation with intersubunit movement.
The complete atomic structure of the large ribosomal subunit at 2.4 A resolution.
The crystal structure of the large ribosomal subunit from Haloarcula marismortui is determined at 2.4 angstrom resolution, and it includes 2833 of the subunit's 3045 nucleotides and 27 of its 31 proteins.
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The structure provides insight into how protein synthesis is initiated and into the evolution of the eukaryotic ribosome, including the function of eIF1 as well as signaling and regulation mediated by the ribosomal proteins RACK1 and rpS6e.
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