The Strong Cell-based Hydrogen Peroxide Generation Triggered by Cold Atmospheric Plasma

  title={The Strong Cell-based Hydrogen Peroxide Generation Triggered by Cold Atmospheric Plasma},
  author={Dayun Yan and Haitao Cui and Wei Zhu and Annie Talbot and Lijie Grace Zhang and Jonathan H Sherman and Michael Keidar},
  journal={Scientific Reports},
Hydrogen peroxide (H2O2) is an important signaling molecule in cancer cells. However, the significant secretion of H2O2 by cancer cells have been rarely observed. Cold atmospheric plasma (CAP) is a near room temperature ionized gas composed of neutral particles, charged particles, reactive species, and electrons. Here, we first demonstrated that breast cancer cells and pancreatic adenocarcinoma cells generated micromolar level H2O2 during just 1 min of direct CAP treatment on these cells. The… 
Universality of Micromolar-Level Cell-Based Hydrogen Peroxide Generation during Direct Cold Atmospheric Plasma Treatment
This study demonstrates the universality of cell-based H2O2 generation that has not been considered in previous studies, and investigates hydrogen peroxide generation at the micromolar level using two CAP-treated cancer cell lines.
The Cell Activation Phenomena in the Cold Atmospheric Plasma Cancer Treatment
The CAP-based cell activation explains the mechanism by which direct CAP treatment causes a much stronger cytotoxicity over the cancer cells compared with an indirect CAP treatment, which is a key to understand what the effect of CAP on cancer cells is.
Dynamics of Singlet Oxygen-Triggered, RONS-Based Apoptosis Induction after Treatment of Tumor Cells with Cold Atmospheric Plasma or Plasma-Activated Medium
The studies allow to conclude that the specific response of tumor cells to generate secondary singlet oxygen is the essential motor for their self-destruction, after a singslet oxygen-mediated triggering process by CAP or PAM.
Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet
Results suggest that among various reactive species produced by PAM, hydrogen peroxide plays a key role in inducing cancer cell apoptosis.
Open-Air Cold Plasma Device Leads to Selective Tumor Cell Cytotoxicity
Plasma cytotoxicity was higher in cells of breast cancer, urinary bladder cancer, osteosarcoma, lung cancer, melanoma, and endometrial cancer, and the results point to the importance of conducting further studies of the therapy with plasma.
Cold plasma-based control of the activation of pancreatic adenocarcinoma cells
A roadmap of control the basic operational parameters of the CAP jet (the helium flow rate, the discharge voltage, and the discharge frequency) on the activation phenomenon is presented, showing the largest impact on theactivation phenomenon.
Cold Atmospheric Plasma Attenuates Breast Cancer Cell Growth Through Regulation of Cell Microenvironment Effectors
The findings of the present study suggest that CAP suppresses breast cancer cell growth and regulates several effectors of the tumor microenvironment and thus it could represent an efficient therapeutic approach for distinct breast cancer subtypes.
Biochemistry of Plasma in Cancer Therapy
This chapter summarizes the role of reactive oxygen/reactive nitrogen species (ROS/RNS) in multistep oncogenesis as well as a selection of central features of the composition and antitumor effects of
Intracellular Responses Triggered by Cold Atmospheric Plasma and Plasma-Activated Media in Cancer Cells
This review discusses the cellular changes appearing in cell membranes, cytoplasm, various organelles, and DNA content upon cells’ direct or indirect exposure to CAP or CAP-activated media/solutions (PAM), respectively.


Induction of apoptosis in human myeloid leukemia cells by remote exposure of resistive barrier cold plasma
  • M. Thiyagarajan, H. Anderson, X. Gonzales
  • Biology
    2014 IEEE 41st International Conference on Plasma Sciences (ICOPS) held with 2014 IEEE International Conference on High-Power Particle Beams (BEAMS)
  • 2014
Assessing the cell death response of human myeloid leukemia cells by remote exposure to CAP generated RNS by utilizing a novel resistive barrier discharge system that primarily produces RNS suggests that CAP emitted RNS play a significant role in the anti-tumor potential of CAP.
An atmospheric-pressure cold plasma leads to apoptosis in Saccharomyces cerevisiae by accumulating intracellular reactive oxygen species and calcium
A non-thermal plasma is known to induce apoptosis of various cells but the mechanism is not yet clear. A eukaryotic model organism Saccharomyces cerevisiaewas used to investigate the cellular and
Production of intracellular reactive oxygen species and change of cell viability induced by atmospheric pressure plasma in normal and cancer cells
The selective effect on cancer and normal cells provides a promising prospect of cold plasma as a cancer therapy.
Toward understanding the selective anticancer capacity of cold atmospheric plasma--a model based on aquaporins (Review).
Preliminary observations indicated that glioblastoma cells consumed H2O2 much faster than did astrocytes in either the CAP-treated or H 2O2-rich media, which supported the selective model based on aquaporins.
The Effect of Tuning Cold Plasma Composition on Glioblastoma Cell Viability
A term “plasma dosage” is defined to summarize the relationship of all the characteristics and cell viability in the plasma jet to determine a mechanism of CAP therapy on glioblastoma cells (U87) through an understanding of the composition of the plasma.
Intracellular effects of atmospheric-pressure plasmas on melanoma cancer cells
It is shown that the plasma treatment induces dose-dependent up-regulation of TP73 gene expression, resulting in significantly elevated levels ofTP73 RNA and protein in plasma-treated melanoma cells, confirming the role of TP 73 protein in dose- dependent regulation of anticancer activity of atmospheric-pressure plasmas.
Atmospheric-Pressure Plasma Jet Induces Apoptosis Involving Mitochondria via Generation of Free Radicals
Antioxidants or caspase inhibitors ameliorated the apoptotic cell death induced by the air and N2 plasma jets, suggesting that the plasma jet may generate ROS as a proapoptotic cue, thus initiating mitochondria-mediated apoptosis.
Altered Antioxidant System Stimulates Dielectric Barrier Discharge Plasma-Induced Cell Death for Solid Tumor Cell Treatment
A promising approach for solid tumor therapy is suggested by delivering a lethal dose of APNT plasma to tumor cells while sparing normal healthy tissues.
Production of large amounts of hydrogen peroxide by human tumor cells.
Constitutive generation of large amounts of reactive oxygen intermediates, if it occurs in vivo, might contribute to the ability of some tumors to mutate, inhibit antiproteases, injure local tissues, and therefore promote tumor heterogeneity, invasion, and metastasis.