The Spatial Localization of T-DNA Insertions in Petunia Interphase Nuclei: Consequences for Chromosome Organization and Transgene Insertion Sites

@article{Hoopen1999TheSL,
  title={The Spatial Localization of T-DNA Insertions in Petunia Interphase Nuclei: Consequences for Chromosome Organization and Transgene Insertion Sites},
  author={Rogier ten Hoopen and Bob M. Montijn and Jacky T. M. Veuskens and O. J. L. Oud and Nanne Nanninga},
  journal={Chromosome Research},
  year={1999},
  volume={7},
  pages={611-623}
}
In an earlier fluorescent in-situ hybridization (FISH) study on petunia (ten Hoopen et al. 1996), we found a considerable discrepancy between the genetic map and the physical map with respect to T-DNA insertions on metaphase chromosomes. For some transgenes we found a preference to integrate near the telomeres. Here, we studied the spatial position of transgenes in interphase nuclei by FISH and 3D-confocal microscopy to elucidate a possible structural preference for the nuclear localization of… CONTINUE READING