The Role of Zinc Ions in Reverse Transport Mediated by Monoamine Transporters*

  title={The Role of Zinc Ions in Reverse Transport Mediated by Monoamine Transporters*},
  author={P. Scholze and L. Norregaard and E. Singer and M. Freissmuth and U. Gether and H. Sitte},
  journal={The Journal of Biological Chemistry},
  pages={21505 - 21513}
  • P. Scholze, L. Norregaard, +3 authors H. Sitte
  • Published 2002
  • Chemistry, Medicine
  • The Journal of Biological Chemistry
  • The human dopamine transporter (hDAT) contains an endogenous high affinity Zn2+ binding site with three coordinating residues on its extracellular face (His193, His375, and Glu396). Upon binding to this site, Zn2+ causes inhibition of [3H]1-methyl-4-phenylpyridinium ([3H]MPP+) uptake. We investigated the effect of Zn2+ on outward transport by superfusing hDAT-expressing HEK-293 cells preloaded with [3H]MPP+. Although Zn2+ inhibited uptake, Zn2+facilitated [3H]MPP+ release induced by amphetamine… CONTINUE READING
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