The Role of Cell Wall Revealed by the Visualization of Saccharomyces cerevisiae Transformation

@article{Pham2010TheRO,
  title={The Role of Cell Wall Revealed by the Visualization of Saccharomyces cerevisiae Transformation},
  author={Tuan Anh Pham and Shigeyuki Kawai and Emi Kono and Kousaku Murata},
  journal={Current Microbiology},
  year={2010},
  volume={62},
  pages={956-961}
}
Transformation is an indispensable method for the manipulation of Saccharomyces cerevisiae cell. The spf1 cell, in which the gene encoding an endoplasmic reticulum-located P-type ATPase is deleted, has been known to show the high-transformation phenotype. In this study, fluorescent microscopic observation of transformation process of S. cerevisiae using plasmid DNA labelled with fluorescent DNA probe, YOYO-1, suggested that the spf1 cell absorbed more plasmid DNA on cellular surface than did… CONTINUE READING