The Role of CRISPR-Cas Systems in Virulence of Pathogenic Bacteria

@article{Louwen2014TheRO,
  title={The Role of CRISPR-Cas Systems in Virulence of Pathogenic Bacteria},
  author={R. Louwen and R. Staals and H. Endtz and P. van Baarlen and J. van der Oost},
  journal={Microbiology and Molecular Biology Reviews},
  year={2014},
  volume={78},
  pages={74 - 88}
}
SUMMARY Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes are present in many bacterial and archaeal genomes. Since the discovery of the typical CRISPR loci in the 1980s, well before their physiological role was revealed, their variable sequences have been used as a complementary typing tool in diagnostic, epidemiologic, and evolutionary analyses of prokaryotic strains. The discovery that CRISPR spacers are often identical to sequence fragments… Expand
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References

SHOWING 1-10 OF 138 REFERENCES
Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system
TLDR
The first examples of genes that mediate the inhibition of a CRISPR/Cas system are described, found in the genomes of bacteriophages infecting Pseudomonas aeruginosa and provides new insight into the co-evolution of phages and bacteria. Expand
A CRISPR-CAS System Mediates Bacterial Innate Immune Evasion and Virulence
TLDR
It is demonstrated that the Cas protein Cas9 of Francisella novicida uses a unique, small, CRISPR/Cas-associated RNA (scaRNA) to repress an endogenous transcript encoding a bacterial lipoprotein. Expand
CRISPR Distribution within the Escherichia coli Species Is Not Suggestive of Immunity-Associated Diversifying Selection
TLDR
The data suggest that, within the E. coli species, CRISPRs do not have the expected characteristics of a classical immune system, and the major phylogenetic group B2 is almost devoid of CRISprs. Expand
Antibiotic resistance plasmids spread among natural isolates of Escherichia coli in spite of CRISPR elements.
TLDR
E. coli CRISPRs do not seem to be efficient barriers to the spread of plasmids and antibiotic resistance, consistent with what has been reported for phages, and contrary to reports concerning other species. Expand
Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli
TLDR
A robust assay in Escherichia coli is described to explore the hitherto least-studied process, adaptation, and essential genes and DNA elements in the leader sequence and in the array which are essential for the adaptation step are identified. Expand
CRISPR‐Cas systems and RNA‐guided interference
  • R. Barrangou
  • Biology, Medicine
  • Wiley interdisciplinary reviews. RNA
  • 2013
TLDR
A number of studies have shown that CRISPR‐mediated immunity can readily increase the breadth and depth of virus resistance in bacteria and archaea and applications of crRNA‐guided interference are discussed. Expand
Envelope stress is a trigger of CRISPR RNA‐mediated DNA silencing in Escherichia coli
TLDR
It is confirmed that the CasCDE enzymes form a stable ternary complex and appear to function as the catalytic core of the Cas system to process CRISPR RNA into its mature form. Expand
Multidrug-Resistant Enterococci Lack CRISPR-cas
TLDR
A highly significant inverse correlation between the presence of a CRISPR-cas locus and acquired antibiotic resistance in E. faecalis suggests that antibiotic use inadvertently selects for enterococcal strains with compromised genome defense. Expand
A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action
TLDR
It appears most likely that CASS is a prokaryotic system of defense against phages and plasmids that functions via the RNAi mechanism, which seems to involve integration of fragments of foreign genes into archaeal and bacterial chromosomes yielding heritable immunity to the respective agents. Expand
Evolution and classification of the CRISPR–Cas systems
TLDR
An updated analysis of the evolutionary relationships between CRISPR–Cas systems and Cas proteins is provided and a 'polythetic' classification that integrates the phylogenies of the most common cas genes, the sequence and organization of theCRISPR repeats and the architecture of the CRISpr–cas loci is proposed. Expand
...
1
2
3
4
5
...